Total Protein (TP)
Why do we do this test?
Measures both albumin and globulins except proteins in the clotting cascade.
1. Coupled TP with albumin give s clue in
hyperglobulinemia ( MGUS, Multiple
myeloma, lymphoma)
2. Low values are seen in primary and
secondary humoral immunodeficiency.
3. Spot urine protein / creatinine ratio and 24
hour urinary protein excretion is done to
assess the urinary protein excretion
4. In CSF protein is done to differete the
pyogenic causes from viral causes of
5. In body fluids especially in plural fluid
protein is done couples with serum protein
to differentiate educate from transudate.
Specimen Type
Serum, Plasma (EDTA), Plasma (Li Heparin), Urine (spot/ timed collection), CSF, Body Fluid
Instructions for specimen collection & Patient preparation
No special preparation is needed
Better blood is drawn without touniquet or touniquet less than 1 minute
Specimen transportation & Processing
Transport in room temperature
Specimen storage & stability
1. At 4°C up to 3 days
2. At -20°C up to 6 months
3. At -70°C long term storage
Specimen storage duration
Reference Intervals & Target Values
65 - 80 g/L
Critical Limits
Possible Pre-analytical Errors
1. Spuriously high values if there is
prolonged stasis during phelebotomy
2. Values of urinary excretion would be
spuriously low or high if not properly
Possible Analytical Errors
Haemolysis 10 g/L might cause positive interference
Icterus causes negative interference
Available Government Laboratories
Recommended turnaround time
1 - 2 hours

Test Method
Blood - Biuret method, Body fluids - dye binding method (Eg. Pyrogallol red)



Last Updated : 2020-09-23 04:19:45






College of Chemical Pathologists of Sri Lanka

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