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Synonyms
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Why do we do this test?
For the diagnosis of cystic fibrosis
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Specimen Type
Sweat sample collected using special sweat stimulation procedure
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Instructions for specimen
collection & Patient preparation
Patient preparation- No special preparation needed, instructed to avoid applying creams or lotions to skin 24 hours & well hydration before the procedure is preferred.
However, the sweat test should be deferred in babies <7 days old and/or <2 kg in weight, subjects who are dehydrated, systemically unwell or who have marked eczema or edema, subjects who are on oxygen by an open delivery system (this does not apply to head-box or nasal prong oxygen)
Specimen collection- Pilocarpine is delivered to a small area of sweat glands on the arm by iontophoresis. The stimulated sweat produced from this area is collected directly into a micro duct for chloride analysis.
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Specimen transportation &
Processing
If analysis is not to be carried out immediately, or if the sample must be transported to a different site for analysis, it is recommended that sweat is transferred to a labeled plain glass capillary tube. An air gap should be left at both ends, which are then sealed with plasticine to prevent evaporation.
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Specimen storage & stability
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Specimen storage duration
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Reference Intervals & Target
Values
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Critical Limits
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Possible Pre-analytical Errors
Contamination of sweat (Chloride containing local anesthetic solutions & skin antiseptics)
Evaporation of sweat during storage
Analysis of sweat sample with inadequate volume
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Possible Analytical Errors
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Available Government Laboratories
Lady Ridge way Hospital for Children- Colombo 08, Chemical pathology laboratory
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Recommended turnaround time
Sweat sample collection by stimulation takes 30-40 minutes.
Report will be available on the same day
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Test Method
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Documentation
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References
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Last Updated : 2020-09-23 03:21:36 |
